STOREDB:STUDY1062 miRNA profile of extracellular vesicles isolated from bone marrow following in vivo irradiation [DOI:10.20348/STOREDB/1062]

Study meta-data


STUDYIDSTOREDB:STUDY1062
CREATEDON2016-12-12 15:26:54
MODIFIEDON2016-12-12 15:34:21
UPLOADERKatalin Lumniczky
DOIDOI:10.20348/STOREDB/1062

Study details


STUDY NAME
miRNA profile of extracellular vesicles isolated from bone marrow following in vivo irradiation
STUDY STATUS
Restricted: Downloaders have to ask uploader for file access
COUNTRY
Hungary
PRINCIPAL INVESTIGATOR
Katalin Lumniczky Ph.D.
SPECIES
Mus musculus
SIZE OF COHORT
0-999
MELODI RESEARCH PRIORITY
Analysis of mechanisms involved in low dose radiation through use and development of suitable cellular and animal models
EXPOSURE CONTEXT
Medical
INTERNAL OR EXTERNAL EXPOSURE
External
TYPE OF EXTERNAL EXPOSURE
X-ray
AGE AT EXPOSURE
Adult
EXPOSURE PATTERN
Acute
DOSE RATE
High
BIOLOGICAL SAMPLE AVAILABLE
No
STUDY DESCRIPTION
Male C57Bl/6 mice were irradiated with low and high dose X-ray irradiation. Mice were sacrificed 24 hours later and bone marrow-derived extracellular vesicles were isolated and microRNAs prepared. A miRNA profiling was performed by Exiqon (Vedbaek, Denmark), using qPCR panel with the miRNA Ready-to-Use PCR, Mouse&Rat panel I+II using ExiLENT SYBRŽ Green master mix. The amplification was performed in a LightCyclerŽ 480 Real-Time PCR System (Roche) in 384 well plates The amplification curves were analysed using the Roche LC software, both for determination of quantification cycles (Cq) (by the 2nd derivative method) and for melting curve (Tm) analysis.

STOREDB:DATASET1101 Raw data microRNA from EV_Szatmari et al [DOI:10.20348/STOREDB/1062/1101]


Created on:2016-12-13 17:30:18 Modified On:2016-12-13 17:35:54
DATASET NAME
Raw data microRNA from EV_Szatmari et al
DOIDOI:10.20348/STOREDB/1062/1101
DATASET DESCRIPTION
miRNA profiling of EVs isolated of bone marrow of whole body irradiated mice. Raw Cq-s of a microRNA qPCR panel are shown.Three experiments were performed (rows A; B; and C), with control (0 Gy), 0.1 Gy and 2 Gy doses.

STOREDB:DATASET1100 Raw data microRNA from EV_Szatmari et al [DOI:10.20348/STOREDB/1062/1100]


Created on:2016-12-12 15:32:32 Modified On:2016-12-12 15:32:32
DATASET NAME
Raw data microRNA from EV_Szatmari et al
DOIDOI:10.20348/STOREDB/1062/1100
DATASET DESCRIPTION
Raw Cq values of miRNA qPCR panel analysis. Three set of samples were profiled: control, irradiated with 0.1 Gy and 2 Gy. Three independent experiments were performed(A-C).