STOREDB:STUDY1000 Integrative cross-omics analysis in cardiac endothelial cells unravels mechanisms of long-term radiation-induced vascular dysfunction [DOI:10.20348/STOREDB/1000]

Study meta-data


STUDYIDSTOREDB:STUDY1000
CREATEDON2015-12-08 19:39:42
MODIFIEDON2016-04-08 12:12:59
UPLOADEROmid Azimzadeh
DOIDOI:10.20348/STOREDB/1000

Study details


STUDY NAME
Integrative cross-omics analysis in cardiac endothelial cells unravels mechanisms of long-term radiation-induced vascular dysfunction
STUDY STATUS
Published: Open access to everyone
DATA SHARING POLICY
CC-Attribution
COUNTRY
Germany
PRINCIPAL INVESTIGATOR
Dr. Omid Azimzadeh
SPECIES
Mus musculus
BIOLOGICAL SAMPLE AVAILABLE
No
STUDY DESCRIPTION
Epidemiological data from radiotherapy patients show the damaging effect of ionising radiation on heart and vasculature. The endothelium is the main target of radiation damage and contributes essentially to the development of cardiac injury. However, the molecular mechanisms behind the radiation-induced endothelial dysfunction are not fully understood. In the present study, 10-week old C57Bl/6 mice received local X-ray heart doses of 8 or 16 Gy and were sacrificed after 16 weeks; the controls were sham-irradiated. The cardiac microvascular endothelial cells were isolated from the heart tissue using streptavidin-CD31 coated micro-beads. The cells were lysed and proteins were labelled with duplex isotope -coded protein label methodology for quantification. All samples were analysed by LC-ESI-MS/MS and Proteome Discoverer software. The proteomics data were further studied by bioinformatics tools and validated by targeted transcriptomics, immunoblotting, immunohistochemistry, and serum profiling. Radiation-induced endothelial dysfunction was characterised by impaired energy metabolism and perturbation of the insulin/IGF-PI3K-Akt signalling pathway. The data also strongly suggested premature endothelial senescence, increased oxidative stress, decreased NO availability and enhanced inflammation as main causes of radiation-induced long-term vascular dysfunction. Detailed data on molecular mechanisms of radiation-induced vascular injury as compiled here are essential in developing radiotherapy strategies that minimise cardiovascular complications.
STOREV2_PROJECTID
http://131.111.32.76/store/project_details.php?projectid=44 (This project has originally been uploaded into the STORE v2 system and is still available here)

STOREDB:DATASET1000 cardiac EC_Highdose_IR [DOI:10.20348/STOREDB/1000/1000]


Created on:2015-12-08 19:40:22 Modified On:2015-12-09 18:31:51
DATASET NAME
cardiac EC_Highdose_IR
DOIDOI:10.20348/STOREDB/1000/1000
DATASET DESCRIPTION
MS/MS data
STOREV2_DATASETID
http://131.111.32.76/store/dataset_details.php?datasetid=48 (This dataset has originally been uploaded into the STORE v2 system and is still available here)