STOREDB:STUDY1041 SUBI tritium exposed Wistar rats [DOI:10.20348/STOREDB/1041]

Study meta-data


STUDYIDSTOREDB:STUDY1041
CREATEDON2016-08-04 16:02:12
MODIFIEDON2016-10-06 14:13:18
UPLOADERBernd Grosche
DOIDOI:10.20348/STOREDB/1041

Study details


STUDY NAME
SUBI tritium exposed Wistar rats
STUDY STATUS
Restricted: Downloaders have to ask uploader for file access
DATA SHARING POLICY
CC-Attribution Non-Commercial
COUNTRY
Russia
PRINCIPAL INVESTIGATOR
K.N. Muksinova
SPECIES
Rattus norvegicus
SIZE OF COHORT
0-999
MELODI RESEARCH PRIORITY
Other activities contributing to low-dose risk research
INTERNAL OR EXTERNAL EXPOSURE
Internal
TYPE OF INTERNAL EXPOSURE
beta
EXPOSURE PATTERN
Protracted
BIOLOGICAL SAMPLE AVAILABLE
Yes
STUDY DESCRIPTION
Information on data and biological material from 224 Wistar rats
held at
Southern Urals Biophysics Institute of the Federal Medical Biological Agency
Ozyersk, Russian Federation

Responsible scientists: Eduard Lubchansky, Olga Kuzmenko, Valentina Revina


Background
The information was gathered from 224 Wistar rats that were used in SUBI’s experiment no. 76323. The collection and collation of the information was done as part of Task 4.8 of the previous EURATOM-funded project DoReMi (Integrating Low Dose Research) [European Atomic Energy Community's Seventh Framework Programme (FP7/2007-2011) under grant agreement n° 249689, coordinated by STUK, Finland; see http://melodi-online.eu/DoReMi/home.html ].
The work was a follow-up of a previous work conducted by SUBI in the frame of EURATOM-funded project STORE (Sustaining access to Tissues and data frOm Radiobiological Experiments; contract number 23228), which was coordinated by BfS, Germany (see http://cordis.europa.eu/project/rcn/89386_en.html) .
The information given here is based upon the final report from SUBI, which can be found in the STORE database.
It has been shown in the STORE project, that the biological material from the experiments conducted by SUBI can still be used. The respective Standard Operating Procedures are available on the STORE website (http://www.storedb.org/store_v3/documents.jsp ).

The SUBI experiment 76323
The study was aimed at investigating late effects in Wistar rats after prolonged (6 months) intake of tritium oxide in a wide range of absorbed doses. It was conducted in the period from 1973 till 1978. The male animals were intragastrically administered with 3.7 (series 1); 37 (series 2) and 370 (series 6) kBq/g/day of tritium oxide on a daily basis during 6 months. Two series of intact rats (93♂) were used as controls for experimental groups (series 4 – control for series 1 and 2; series 7 – control for series 6). The processing of organs and tissues of the animals which died natural death included: fixation in formalin; washing; dehydration in spirits and holding in castor oil with celloidin; paraffin embedding; preparation of blocks; preparation of cuts for microscopy. The procedure is presented in detail in the file SUBI_Paraffin_Block_Preparation.pdf.

Information on individual animals
The information on the individual animals is very detailed and given in Russian.

Available biomaterial
Paraffin-fixed samples of organs and tissues are available for 224 rats of all 5 series. They include: lungs (1080 samples); heart (221); stomach (217); small (737) and large (396) intestine; pancreas (193); liver (247); kidneys (434); bladder (71); bone marrow (229); lymph nodes, mostly mesentery (335); spleen (217); hypophysis (210); adrenals (431); thyroid (383) and parathyroid (358) glands; testicles (401); decalcified bones of different part of the skeleton (1883), as well as 49 samples of tumor diseases.
An overview on the number of organs per study group is available in.

Contact information
If you want to use the material you should contact SUBI through inter_dep@subi.su. If you have want to have access to the individual data or have any questions regarding this short summary report or SUBI’s final report, please contact Dr. Bernd Grosche (bgrosche@t-online.de).

STOREDB:DATASET1071 Series 4 [DOI:10.20348/STOREDB/1041/1071]


Created on:2016-08-09 12:17:37 Modified On:2016-08-09 12:17:37
DATASET NAME
Series 4
DOIDOI:10.20348/STOREDB/1041/1071
DATASET DESCRIPTION
Information on individual animals from Series 4