STOREDB:STUDY1042 Combined effects of Pu-239 Dioxide and Cigarette Smoke on the Production of Lung Tumours in the Mouse [DOI:10.20348/STOREDB/1042]

Study meta-data


STUDYIDSTOREDB:STUDY1042
CREATEDON2016-09-12 12:58:45
MODIFIEDON2016-09-12 12:58:45
UPLOADERMichael Gruenberger
DOIDOI:10.20348/STOREDB/1042

Study details


STUDY NAME
Combined effects of Pu-239 Dioxide and Cigarette Smoke on the Production of Lung Tumours in the Mouse
STUDY STATUS
Published: Open access to everyone
COUNTRY
United Kingdom
PRINCIPAL INVESTIGATOR
Dr. Nick Priest
SPECIES
Mus musculus
BIOLOGICAL SAMPLE AVAILABLE
No
STUDY DESCRIPTION
Purpose: To determine whether cigarette smoke and Pu-239 dioxide act synergistically with respect to the production of lung tumours in CBA/Ca mice.

Status: 1987 - 1991, terminated

Treatment: Actinide exposure: single nose-only inhalation of Pu-239 (1.5 m AMAD, 1.2-1.3 g) prepared by calcination of the oxalate at 550oC for 3 hours.

Smoke exposure: Nose-only inhalation of mainstream smoke generated from high-tar un-tipped cigarettes diluted 40-fold with clean air (tar particulate =1.4 mg/l, CO concentration =1000 ppm). Twice-daily 30 minute exposures, 5 days a week for 12 months.

A Dose effect relationship

B Effect of smoking

Dosimetry: Radiochemical analysis to determine Pu239 content of lungs from mice killed at 1, 7, 28, 84, 196 and 364 days post actinide exposure. Trapezoidal method to calculate average radiation dose to lungs.

Endpoints: Terminal sacrifice 18 months after actinide exposure, plus sporadic deaths. Necropsy observation and histopathology of all macroscopically obvious abnormalities. Lungs cleared to determine the absolute number of lung lesions.

Animal: Female CBA/Ca mice 10 weeks of age (approximately 20 g) at time of actinide exposure.

Results: The study failed to demonstrate a synergistic effect on the production of lung tumours following the exposure of CBA/Ca mice to the combined insult of Pu-239 and cigarette smoke. The results did, however, indicate an apparent effect of stress on the tumour frequency, as animals that were sham-smoked also had a lower incidence of lung tumours compared to cage-controls given the same dose of plutonium.

STOREDB:DATASET1076 Link to data and details in ERA [DOI:10.20348/STOREDB/1042/1076]


Created on:2016-09-12 12:59:35 Modified On:2016-09-12 12:59:35
DATASET NAME
Link to data and details in ERA
DOIDOI:10.20348/STOREDB/1042/1076
LINK TO FILE
https://era.bfs.de/studies_details.php?LabId=1&StudyId=1