STOREDB:STUDY1045 Effects of Radiation Quality on the Induction of Leukaemia in CBA/CA Mice [DOI:10.20348/STOREDB/1045]

Study meta-data


STUDYIDSTOREDB:STUDY1045
CREATEDON2016-09-29 18:09:46
MODIFIEDON2016-09-29 18:09:46
UPLOADERMichael Gruenberger
DOIDOI:10.20348/STOREDB/1045

Study details


STUDY NAME
Effects of Radiation Quality on the Induction of Leukaemia in CBA/CA Mice
STUDY STATUS
Published: Open access to everyone
COUNTRY
United Kingdom
PRINCIPAL INVESTIGATOR
Dr. Jeremy Kellington
SPECIES
Mus musculus
BIOLOGICAL SAMPLE AVAILABLE
No
STUDY DESCRIPTION
Purpose: To study the induction of myeloid leukaemias in mice following the administration of fused aluminosilicate particles (FAP) incorporating aloha- and beta-emitting radionuclides. This information will be used to estimate the most appropriate value for the quality factor of high LET radiations with respect to radiation-induced haemopoietic malignancies.

Status: 1991 - 1995

Treatment: Single intravenous administration of fused aluminosilicate particles (FAP) labelled with Cm-242 (alpha-emitter) or Ca-45 (beta-emitter). Actual diameter of FAP =1.90 0.85 μm.

Dosimetry: Radiochemical analysis to determine Cm-242 or Ca-45 content of tissues from mice killed at 1, 7, 14, 30, 76, 150, 300 and 601 days post injection. Trapezoidal method used to calculate average radiation dose to tissues. Also autoradiographic analysis of liver, spleen and bone marrow to determine microdistribution of FAP.

Endpoints: Life-span study with sacrifice of moribund animals. Necropsy observation and histopathology of all macroscopically obvious abnormalities. Also full haematology for all animals killed in extremis

Animal: Female CBA/Ca mice 10 weeks of age (approximately 20 g) at time of exposure

Results: Following injection with the highest levels of Cm-242 or Ca-45 FAPs, survival was reduced by about 4 and 3 months respectively from the 900 days lifespan of controls . Liver tumours were observed in about 10% of the controls but in about 20% of Cm242-injected mice. Subcutaneous masses thought to arise from benign enlargement of subcutaneous lymph nodes were the most common (30%) abnormality in controls and contributed to morbidity and mortality of the animals. However, the incidence of these masses was reduced in animals injected with the highest levels of Cm-242 or Ca-45 FAPs. On the contrary the incidence of animals with enlargement of multiple lymph nodes, splenomegaly and accumulation of fluid in abdomen and thoracic cavity, signs characteristic of leukaemias, increased from low levels in controls to 11% following injection of radio-labelled FAPs. The histopathological evaluation is now under way.

STOREDB:DATASET1079 Link to data and details in ERA [DOI:10.20348/STOREDB/1045/1079]


Created on:2016-09-29 18:10:06 Modified On:2016-09-29 18:10:06
DATASET NAME
Link to data and details in ERA
DOIDOI:10.20348/STOREDB/1045/1079
LINK TO FILE
https://era.bfs.de/studies_description_from_db.php?LabId=1&StudyId=4