STOREDB:STUDY1096 Radiation-induced Endothelial Inflammation is Transferred via Secretome to Recipient Cells in a STAT-Driven Process [DOI:10.20348/STOREDB/1096]

Study meta-data


STUDYIDSTOREDB:STUDY1096
CREATEDON2017-06-09 15:17:12
MODIFIEDON2017-06-22 16:49:37
UPLOADERJos
DOIDOI:10.20348/STOREDB/1096

Study details


STUDY NAME
Radiation-induced Endothelial Inflammation is Transferred via Secretome to Recipient Cells in a STAT-Driven Process
STUDY STATUS
Published: Open access to everyone
DATA SHARING POLICY
CC-Attribution Non-Commercial Share Alike
COUNTRY
Germany
PRINCIPAL INVESTIGATOR
Philipp, Jos
SPECIES
Homo sapiens
SIZE OF COHORT
0-999
OUTCOME
Cardiovascular
MELODI RESEARCH PRIORITY
Examination of the mechanisms and effects of mixed and multi-exposure on cells, tissues, animal models and humans.
EXPOSURE CONTEXT
Medical
INTERNAL OR EXTERNAL EXPOSURE
External
TYPE OF EXTERNAL EXPOSURE
X-ray
AGE AT EXPOSURE
Adult
EXPOSURE PATTERN
Acute
DOSE RATE
High
BIOLOGICAL SAMPLE AVAILABLE
No
STUDY DESCRIPTION
Irradiation is the most common treatment of cancer. However, minimizing the normal tissue injury, especially the damage to vascular endothelium, still remains a challenge. This study aimed to analyze direct and indirect radiation effects by investigating mechanisms of signal transfer from irradiated to non-irradiated endothelial cells by means of secreted proteins. It is known that human coronary artery endothelial cells (HCAECest2) undergo radiation-induced senescence 14 days post-exposure to 10 Gy X-rays. Proteomics analysis was performed using label-free technology on HCAECest2 at this time point after irradiation with X-ray doses of 0 Gy (control) or 10 Gy. Additionally, the proteomes of control and radiation-induced secretomes, and those of non-irradiated HCAECest2 exposed for 24 hours to secreted proteins of either condition were measured. Key changes identified by proteomics and bioinformatics were validated by immunoblotting, ELISA, bead-based multiplex assays, and targeted transcriptomics. The irradiated cells, their secretome and the non-irradiated recipient cells showed inflammatory response, characterized by induction of interferon type I-related proteins and activation of STAT3 pathway. These data show that radiation-induced premature senescence in endothelial cells exerts detrimental effects via senescence-associated secretory phenotype on the non-irradiated microenvironment. This study adds to our knowledge of the pathological background of radiation-induced cardiovascular disease.
MEAN DURATION OF FOLLOW-UP (WEEKS)
2

STOREDB:DATASET1136 Irradiated and Recipient replicates + Secretome [DOI:10.20348/STOREDB/1096/1136]


Created on:2017-06-09 15:21:02 Modified On:2017-06-22 14:54:35
DATASET NAME
Irradiated and Recipient replicates + Secretome
DOIDOI:10.20348/STOREDB/1096/1136
DATASET DESCRIPTION
Uploaded raw data of the irradiated and recipient replicates. Additionally also the raw files of the secretome runs were added.
Files are named by the running number on the MS-machine, followed by the Replicate number and the internal abbreviation. The treatment is mentioned thereafter (irradiated, recipient, secretome and the used dose)
STOREDB:FILE11219

AZO8610A12MA2 Replicate3-C3 DonorSecretome 10 Gy

Uploaded on: 2017-06-22 14:33:01
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11218

AZO8610A10MA2 Replicate2-C2 DonorSecretome 10 Gy

Uploaded on: 2017-06-22 14:24:00
Type: application/x-7z-compressed
File size: 998 megabytes
STOREDB:FILE11217

AZO8610A8MA2 Replicate1-C1 DonorSecretome 10 Gy

Uploaded on: 2017-06-22 14:17:02
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11216

AZO8610A6MA2 Replicate3-A3 DonorSecretome 0 Gy

Uploaded on: 2017-06-21 09:33:09
Type: application/x-7z-compressed
File size: 996 megabytes
STOREDB:FILE11215

AZO8610A4MA2 Replicate2-A2 DonorSecretome 0 Gy

Uploaded on: 2017-06-21 09:27:38
Type: application/x-7z-compressed
File size: 989 megabytes
STOREDB:FILE11214

AZO8610A2MA3 Replicate1-A1 DonorSecretome 0 Gy

Uploaded on: 2017-06-21 09:19:28
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11213

AZO8607A24MA4 Replicate3-D3 Recipient 10 Gy

Uploaded on: 2017-06-21 09:12:09
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11212

AZO8607A22MA4 Replicate2-D2 Recipient 10 Gy

Uploaded on: 2017-06-21 09:03:53
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11211

AZO8610A12MA2 Replicate3-C3 DonorSecretome 10 Gy

Uploaded on: 2017-06-19 18:06:04
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE11210

AZO8610A10MA2 Replicate2-C2 DonorSecretome 10 Gy

Uploaded on: 2017-06-19 17:54:15
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE11209

AZO8610A8MA2 Replicate1-C1 DonorSecretome 10 Gy

Uploaded on: 2017-06-19 17:44:13
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE11208

AZO8610A6MA2 Replicate3-A3 DonorSecretome 0 Gy

Uploaded on: 2017-06-19 17:33:23
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE11207

AZO8610A4MA2 Replicate2-A2 DonorSecretome 0 Gy

Uploaded on: 2017-06-19 17:21:36
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE11206

AZO8610A2MA3 Replicate1-A1 DonorSecretome 0 Gy

Uploaded on: 2017-06-19 17:05:28
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE11205

AZO8607A24MA4 Replicate3-D3 Recipient 10 Gy

Uploaded on: 2017-06-19 16:50:38
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11204

AZO8607A22MA4 Replicate2-D2 Recipient 10 Gy

Uploaded on: 2017-06-19 16:37:40
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11203

AZO8607A20MA4 Replicate1-D1 Recipient 10 Gy

Uploaded on: 2017-06-19 16:24:41
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11199

AZO8607A18MA2 Replicate3-C3 Irradiated 10 Gy

Uploaded on: 2017-06-19 16:12:36
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11198

AZO8607A16MA2 Replicate2-C2 Irradiated 10 Gy

Uploaded on: 2017-06-19 16:01:25
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11197

AZO8607A14MA2 Replicate1-C1 Irradiated 10 Gy

Uploaded on: 2017-06-19 15:30:47
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11196

AZO8607A12MA4 Replicate3-B3 Recipient 0 Gy

Uploaded on: 2017-06-19 15:15:02
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11195

AZO8607A10MA4 Replicate2-B2 Recipient 0 Gy

Uploaded on: 2017-06-19 15:00:56
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11194

AZO8607A8MA4 Replicate1-B1 Recipient 0 Gy

Uploaded on: 2017-06-19 14:33:18
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11193

AZO8607A4MA2 Replicate2-A2 Donor 0 Gy

Uploaded on: 2017-06-19 14:18:13
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11192

AZO8607A6MA2 Replicate3-A3 Donor 0 Gy

Uploaded on: 2017-06-19 14:01:36
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11191

AZO8607A2MA3 Replicate1-A1 Donor 0 Gy

Uploaded on: 2017-06-19 13:47:16
Type: image/x-panasonic-raw
File size: 2 gigabytes
STOREDB:FILE11189

AZO8607A20MA4 Replicate1-D1 Recipient 10 Gy

Uploaded on: 2017-06-19 13:04:33
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11188

AZO8607A18MA2 Replicate3-C3 Irradiated 10 Gy

Uploaded on: 2017-06-19 12:56:44
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11187

AZO8607A16MA2 Replicate2-C2 Irradiated 10 Gy

Uploaded on: 2017-06-19 12:47:37
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11186

AZO8607A14MA2 Replicate1-C1 Irradiated 10 Gy

Uploaded on: 2017-06-19 12:37:19
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11185

AZO8607A12MA4 Replicate3-B3 Recipient 0 Gy

Uploaded on: 2017-06-19 11:59:19
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11184

AZO8607A10MA4 Replicate2-B2 Recipient 0 Gy

Uploaded on: 2017-06-19 11:50:04
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11183

AZO8607A8MA4 Replicate1-B1 Recipient 0 Gy

Uploaded on: 2017-06-19 11:41:22
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11182

AZO8607A6MA2 Replicate3-A3 Donor 0 Gy

Uploaded on: 2017-06-19 11:31:46
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11181

AZO8607A4MA2 Replicate2-A2 Donor 0 Gy

Uploaded on: 2017-06-19 11:21:40
Type: application/x-7z-compressed
File size: 1 gigabytes
STOREDB:FILE11180

AZO8607A2MA3 Replicate1-A1 Donor 0 Gy

Uploaded on: 2017-06-19 11:13:23
Type: application/x-7z-compressed
File size: 1 gigabytes