| STUDYID | STOREDB:STUDY1096 |
|---|---|
| CREATEDON | 2017-06-09 15:17:12 |
| MODIFIEDON | 2017-06-22 16:49:37 |
| UPLOADER | Jos |
| DOI | DOI:10.20348/STOREDB/1096 |
| STUDY NAME | ||
|---|---|---|
| Radiation-induced Endothelial Inflammation is Transferred via Secretome to Recipient Cells in a STAT-Driven Process | ||
| STUDY STATUS | ||
| Published: Open access to everyone | ||
| DATA SHARING POLICY | ||
| CC-Attribution Non-Commercial Share Alike | ||
| COUNTRY | ||
| Germany | ||
| PRINCIPAL INVESTIGATOR | ||
| Philipp, Jos | ||
| SPECIES | ||
| Homo sapiens | ||
| SIZE OF COHORT | ||
| 0-999 | ||
| OUTCOME | ||
| Cardiovascular | ||
| RADONORM RESEARCH PRIORITY | ||
| Examination of the mechanisms and effects of mixed and multi-exposure on cells, tissues, animal models and humans. | ||
| EXPOSURE CONTEXT | ||
| Medical | ||
| INTERNAL OR EXTERNAL EXPOSURE | ||
| External | ||
| TYPE OF EXTERNAL EXPOSURE | ||
| X-ray | ||
| AGE AT EXPOSURE | ||
| Adult | ||
| EXPOSURE PATTERN | ||
| Acute | ||
| DOSE RATE | ||
| High | ||
| BIOLOGICAL SAMPLE AVAILABLE | ||
| No | ||
| STUDY DESCRIPTION | ||
| Irradiation is the most common treatment of cancer. However, minimizing the normal tissue injury, especially the damage to vascular endothelium, still remains a challenge. This study aimed to analyze direct and indirect radiation effects by investigating mechanisms of signal transfer from irradiated to non-irradiated endothelial cells by means of secreted proteins. It is known that human coronary artery endothelial cells (HCAECest2) undergo radiation-induced senescence 14 days post-exposure to 10 Gy X-rays. Proteomics analysis was performed using label-free technology on HCAECest2 at this time point after irradiation with X-ray doses of 0 Gy (control) or 10 Gy. Additionally, the proteomes of control and radiation-induced secretomes, and those of non-irradiated HCAECest2 exposed for 24 hours to secreted proteins of either condition were measured. Key changes identified by proteomics and bioinformatics were validated by immunoblotting, ELISA, bead-based multiplex assays, and targeted transcriptomics. The irradiated cells, their secretome and the non-irradiated recipient cells showed inflammatory response, characterized by induction of interferon type I-related proteins and activation of STAT3 pathway. These data show that radiation-induced premature senescence in endothelial cells exerts detrimental effects via senescence-associated secretory phenotype on the non-irradiated microenvironment. This study adds to our knowledge of the pathological background of radiation-induced cardiovascular disease. | ||
| MEAN DURATION OF FOLLOW-UP (WEEKS) | ||
| 2 | ||
| DATASET NAME | ||
|---|---|---|
| Irradiated and Recipient replicates + Secretome | ||
| DOI | DOI:10.20348/STOREDB/1096/1136 | |
| DATASET DESCRIPTION | ||
| Uploaded raw data of the irradiated and recipient replicates. Additionally also the raw files of the secretome runs were added.
Files are named by the running number on the MS-machine, followed by the Replicate number and the internal abbreviation. The treatment is mentioned thereafter (irradiated, recipient, secretome and the used dose) | ||
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