STOREDB:STUDY1153 CREB signaling mediates dose-dependent radiation response in the murine hippocampus two years after low-dose total body exposure [DOI:10.20348/STOREDB/1153]

Study meta-data


STUDYIDSTOREDB:STUDY1153
CREATEDON2019-07-23 13:15:23
MODIFIEDON2019-08-26 12:50:13
UPLOADERDaniela Hladik
DOIDOI:10.20348/STOREDB/1153

Study details


STUDY NAME
CREB signaling mediates dose-dependent radiation response in the murine hippocampus two years after low-dose total body exposure
STUDY STATUS
Published: Open access to everyone
COUNTRY
Germany
SPECIES
Mus musculus
SIZE OF COHORT
0-999
OUTCOME
Cognitive effects
INTERNAL OR EXTERNAL EXPOSURE
External
TYPE OF EXTERNAL EXPOSURE
gamma
RADIONUCLIDE
caesium
AGE AT EXPOSURE
Adult
EXPOSURE PATTERN
Acute
DOSE RATE
Low
BIOLOGICAL SAMPLE AVAILABLE
No
ECOLOGICAL DATA
No
STUDY DESCRIPTION
The impact of low-dose ionizing radiation (IR) on human brain has recently attracted attention due to increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in hippocampus. Female C57Bl/6 mice were irradiated with 0, 0.063, 0.125 or 0.5 Gy and quantitative label-free proteomic analysis of hippocampus was performed after 24 months. Key pathways were validated by immunoblotting. CREB signaling and CREB-associated pathways were the most affected ones at all doses. The two lower doses seemed to induce CREB pathway, whereas the exposure to 0.5 Gy deactivated CREB. Similarly, the lowest dose (0.063 Gy) was anti-inflammatory reducing the number of activated microglia (IBA-1), whilst induction of activated microglia and reactive astroglia (GFAP) was found at 0.5 Gy suggesting increased inflammation and astrogliosis, respectively. Apoptotic markers BAX and cleaved CASP-3 and oxidative stress markers (carbonylation, NRF-2) were increased only at the highest dose. Since activated CREB pathway plays a central role in learning and memory, these data suggest neuroprotection at the lowest dose (0.063 Gy) but neurodegeneration at 0.5 Gy. These effects become significant only in old animals (24 m) and support the hypothesis of radiation-induced accelerated aging in the brain.

STOREDB:DATASET1215 Raw files [DOI:10.20348/STOREDB/1153/1215]


Created on:2019-08-22 10:55:19 Modified On:2019-08-22 10:58:58
DATASET NAME
Raw files
DOIDOI:10.20348/STOREDB/1153/1215
DATASET DESCRIPTION
MS spectral information

STOREDB:DATASET1214 Lists of all identified proteins of the 4, 12 and 18 months timepoint [DOI:10.20348/STOREDB/1153/1214]


Created on:2019-08-13 13:33:51 Modified On:2019-08-13 13:33:51
DATASET NAME
Lists of all identified proteins of the 4, 12 and 18 months timepoint
DOIDOI:10.20348/STOREDB/1153/1214