STOREDB:STUDY1168 Hypothermia differentially modulates the formation and decay of 53BP1 and NBS1 foci in U2OS cells exposed to gamma radia-tion [DOI:10.20348/STOREDB/1168]

Study meta-data


STUDYIDSTOREDB:STUDY1168
CREATEDON2021-03-16 13:56:29
MODIFIEDON2021-03-16 13:56:29
UPLOADERAndrzej Wojcik
DOIDOI:10.20348/STOREDB/1168

Study details


STUDY NAME
Hypothermia differentially modulates the formation and decay of 53BP1 and NBS1 foci in U2OS cells exposed to gamma radia-tion
STUDY STATUS
Restricted: Downloaders have to ask uploader for file access
COUNTRY
Poland
PRINCIPAL INVESTIGATOR
Magdalena Płódowska
SPECIES
Homo sapiens
INTERNAL OR EXTERNAL EXPOSURE
External
TYPE OF EXTERNAL EXPOSURE
gamma
EXPOSURE PATTERN
Acute
DOSE RATE
High
BIOLOGICAL SAMPLE AVAILABLE
No
ECOLOGICAL DATA
No
STUDY DESCRIPTION
In studies on the mechanism of DNA damage response where ionizing radiation is used as the DNA damaging agent, cells are often exposed to ionizing radiation on melting ice (corresponding to 0.8°C). The purpose of this procedure is to inhibit cellular processes i.e. DNA repair. Low temperature at exposure has been shown to act in a radioprotective manner at the level of cytogenetic damage, but its mechanisms of action are poorly understood. The aim of the study was to analyze the effect of hypothermia at the level of formation and decay of 53BP1 and NBS1 foci and micronuclei in U2OS cells. The results show that hypothermia alone induced foci and irradiation lead to an increased formation of 53BP1 but not of NBS1 foci. Hypothermia dur-ing irradiation and subsequent repair at 0.8°C showed that 53BP1 and NBS1 foci formed and disappeared, but at levels markedly lower than at 37°C. The frequency of micronuclei was de-tected in cells irradiated at 0.8°C, but only 20h after irradiation. The temperature effect should be considered when cooling cells on melting ice to inhibit DNA repair in the induction of DNA damage.

STOREDB:DATASET1242 Plodowska et al data.xlsx [DOI:10.20348/STOREDB/1168/1242]


Created on:2021-03-16 13:59:21 Modified On:2021-03-16 13:59:21
DATASET NAME
Plodowska et al data.xlsx
DOIDOI:10.20348/STOREDB/1168/1242
DATASET DESCRIPTION
Excel file with numerical data corresponding to figures in the manuscript